Journal: Nature Communications
Article Title: Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cells
doi: 10.1038/s41467-025-60474-1
Figure Lengend Snippet: Olfm4 flox/flox mice with Ucp1 -Cre mice were crossed to construct brown adipocyte-specific knockout mice ( Ucp1 -Cre + ; Olfm4 flox/flox , Olfm4CKO) for Olfm4 , and littermate Cre-negative mice ( Ucp1 -Cre - ; Olfm4 flox/flox , Ctrl) served as controls. Ctrl and Olfm4CKO mice (8 weeks old) were treated with normal diet (ND), Body weight was recorded ( n = 6 mice per group) ( A ). General representative image and weight of Ctrl and Olfm4CKO iBAT ( B ). Hematoxylin-eosin (HE) staining of Ctrl and Olfm4CKO iBAT ( n = 6 mice per group) ( C ) (scale bars, 50 μm and 20 μm). Triglyceride quantification of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( D ). Indirect calorimetry analysis of Ctrl and Olfm4CKO mice, monitored over a 24 h period ( n = 3 mice per group) ( E ). Daily food intake of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( F ). Ctrl and Olfm4CKO mice were cold challenged at 4 °C. Rectal temperatures are shown ( n = 6 mice per group) ( G ). Ctrl and Olfm4CKO mice (8 weeks old) were treated with an high-fat diet (HFD) for 10 weeks. Body weight was recorded each week ( n = 6 mice per group) ( H ). Tissues were collected and weighed. Fat mass of Ctrl and Olfm4CKO mice ( n = 6 mice per group) ( I ). Lean mass of Ctrl and Olfm4CKO mice ( n = 6 mice per group) (J). Intraperitoneal glucose tolerance test (GTT) ( n = 6 mice per group) ( K ). Histology of iBAT ( L ) and liver ( M ) of Ctrl and Olfm4CKO mice on an HFD (scale bars, 50 μm and 20 μm). All Data are mean ± s.e.m. of biologically independent samples. Statistical analysis was performed using two-tailed unpaired Student’s t -test ( A − J ) and two-tailed unpaired Welch’s t -test ( G − K ). All experiments were independently repeated at least three times with similar results. Source data are provided as a Source Data file.
Article Snippet: The concentration of triglycerides in the serum was quantified with a serum triglyceride determination kit (#98-11086-01, IDEXX, Westbrook, USA) and a seralyzer (Catalyst Dx TM , IDEXX, Westbrook, USA).
Techniques: Construct, Knock-Out, Staining, Two Tailed Test
